CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars

Abstract Background Filamentous fungi are excellent lignocellulose degraders, which they achieve through producing carbohydrate active enzymes (CAZymes). CAZyme production is highly orchestrated and gene expression analysis has greatly expanded understanding of this important biotechnological process. The thermophilic fungus Thermoascus aurantiacus secretes thermostable that enable saccharifications at higher temperatures; however, the genome-wide measurements in response to induction not understood. Results A fed-batch system with plant biomass-derived sugars d -xylose, l -arabinose cellobiose established these induce T. . C5 induced both cellulases hemicellulases, while specifically cellulases. minimal medium formulation was developed studies inducers. It found d-xylose strongly a wide variety CAZymes, auxiliary activity (AA) esterases (CEs), facilitated lower mostly cellulase genes. Furthermore, putative orthologues different unfolded protein genes were up-regulated during sugar feeding together assimilation pathways. Conclusion This work identified two additional inducers for , cellobiose, along -xylose. combination biochemical assays RNA-seq suite providing paths produce broad spectrum thermotolerant enzymatic mixtures.